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KMID : 0613820190290111227
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Journal of Life Science
2019 Volume.29 No. 11 p.1227 ~ p.1234
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Inhibitory Effects of Prunus mume Solvent Fractions on Human Colon Cancer Cells
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Kim Jeong-Ho
Cho Hyun-Dong
Won Yeong-Seon
Heo Ji-An
Kim Ji-Young
Kim Hwi-Gon
Han Sim-Hee
Moon Kwang-Deog
Seo Kwon-Il
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Abstract
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Prunus mume, also known as maesil, is a popular fruit consumed in East Asia (Korea, Japan, and China). It contains high amounts of organic acids, minerals, and polyphenols and has been used as a medication for fever, vomiting, and detoxification. In this study, the anti-proliferative and apoptotic effects of solvent fractions from maesil were evaluated using sulforhodamine B (SRB) assays, morphological evaluations, Hoechst 33258 staining, and western blotting. Addition of the maesil methanol fraction (MMF) and the maesil butanol fraction (MBF) significantly and dose-dependently decreased the cell viability of HT-29 human colon cancer cells. Colony-forming assays confirmed that the MMF and MBF treatments decreased colony numbers when compared with untreated control cells. Treatment of HT-29 cells with MMF and MBF caused a distortion of the cell morphology to a shrunken cell mass. Treatment with MMF and MBF also dose-dependently increased nuclear condensation and the formation of apoptotic bodies in HT-29 cells. Treatment with MMF and MBF significantly and dose- dependently increased the expression of Bax (a pro-apoptotic protein), caspase-3, and poly ADP-ribose polymerase (PARP) and decreased the expression of Bcl-2 (an anti-apoptotic protein). MMF significantly and dose-dependently inhibited cell proliferation induced by bisphenol A, an environmental hormone. Therefore, MMF may have potential use as a functional food and as a possible therapeutic agent for the prevention of colon cancer.
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KEYWORD
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Apoptosis, colon cancer, environmental hormone, Prunus mume, solvent fraction
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